Volume : 10, Issue : 02, February – 2023

Title:

03.ANTIBIOTIC SUSCEPTIBILITY PATTERN OF BACTERIAL ISOLATES FROM SURFACES OF VARIOUS REFRIGERATED AND NON-REFRIGERATED CANNED DRINKS SOLD WITHIN ABAKALIKI METROPOLIS
‬

Authors :

Okonkwo Eucharia Chinyere, Ndukwe Emmanuel Chisom, Agah Maduka Victor, Idioha Jude Chinedu and Nwachi Anthonia Chinyere.

Abstract :

The antibiotic susceptibility profile of bacterial isolates from the surfaces of various refrigerated and non-refrigerated canned drinks sold within Abakaliki metropoli were evaluated in this study. Standard bacteriological culture-based techniques, employing the use of media including: Nutrient broth, Nutrient agar, Mannitol salt agar, Cetrimide agar, Salmonella-Shigella agar and MacConkey agar were used for culture and isolation of bacteria while microscopy and biochemical analysis were employed for identification. A total of 80 canned drinks surfaces were swabbed and subjected to analysis. Kirby-Bauer disc diffusion technique was used for antibacterial susceptibility testing. The multiple antibiotic resistance index (MARI) was deduced from the antibiogram characterization to evaluate the public health importance of the bacterial isolates. Refrigerated samples had a frequency occurrence of 28.6% contamination while 71.4% contamination was observed for non-refrigerated samples. The bacterial species isolated include Staphylococcus aureus 14 (40%), Pseudomonas aeruginosa 8 (23.8%), Escherichia coli 10 (28.6%) and Salmonella species 3 (8.6%). The isolates were most susceptible to Ciprofloxacin (93.8%) and Gentamicin (85.2%) and least susceptible to Cefixime (22.1%) and Cefuroxime (30.3%). The outcome of the investigation revealed that the surfaces of canned drinks sold in many open markets to both direct and indirect consumers are possibly contaminated with microorganisms. It is recommended to clean the top surfaces of canned drinks before drinking to reduce risk of bacterial contaminants.
Key words: Antibiotic, Refrigerated, Non-refrigerated, Canned Drinks, contamination

Cite This Article:

Please cite this article in press Okonkwo Eucharia Chinyere et al, Antibiotic Susceptibility Pattern Of Bacterial Isolates From Surfaces Of Various Refrigerated And Non-Refrigerated Canned Drinks Sold Within Abakaliki Metropolis., Indo Am. J. P. Sci, 2023; 10(02).

Number of Downloads : 10

References:

1. Bauer, AW, Kirby, MMW, Sherris, JC and Turck, M. Antibiotics susceptibility testing by astandardized single disk method. Am. J. Clin. Pathol.,2006 36: 493–496
2. Chitanand, MP, Kadam, TA, Gyananath, G, Totewad, ND and Balhal, DK., Multiple antibiotic resistance indexing of coliforms to identify high risk contamination sites in aquatic environment. Ind.J. Microbiol 2010 50:216–220.
3. Dantas, ST, Silva, N., Dantas, FBH. External Microbiological Contamination of Beverage Packaging. Brazil. J. FoodTech 2006. 93, 193-199.
4. Ekrami, AR., Kayedani, A, Jahangir, M, Kalantar, E. and Jalali, M. Isolation of common aerobic bacterial pathogens from the environment of seven hospitals,Ahvaz, Iran. Jundishapur J.Microbiol 2010.,42, 75-82.
5. Kigigha, LT. and Jonathan, G., Microbiological assessment of opened soft drink bottles for pathogenic bacteria associated with drinking directly from the orifice. Cont. J. Microbiol 2012 61, 26 – 32.
6. Kilic, LH., Ozaslan, M, Karagoz, ID., Zer, Y. and Davutoglu, V. The microbial colonization of mobile phone used by healthcare staff.Biol. J. Biol. Sci 2009., 121: 882-884.
7. Kusumaningrum, HD., Riboldi, G., Hazeleger, WC. and Beumer, RR., Survival of food borne pathogens on stainless steel surfaces and cross-contamination to foods.Int. J. FoodMicrobiol 2002., 85, 227–236.
8. Michaels B, Ganger V, Schultz A, Curiale MS. 2003. A microbial survey of food service can openers food and beverage can tops and cleaning methodology effectiveness. FoodServiceTechnology., 3: 123-132.
9. Ogofure, AG., Bello-Osagie, OI.,Aduba, UB., Ighodaro, EV. and Emoghene, AO. Qualitative detection and isolation of bacteria from the surfaces of canned drinks sold in Ugbor, Benin City. Annals of Science and Technology, 20183(2): 20-25.
10. Otu-Bassey, IB., Ewaoche, IS., Okon, FB. and Ibor, UA. .Microbial Contamination ofHouse Hold Refrigerators in Calabar Metropolis- Nigeria. Am. J. Epidem. Infect. Dis. 2017 ,51, 1-7