Volume : 10, Issue : 06, June – 2023

Title:

41.ANALYTICAL METHOD DEVELOPMENT AND METHOD VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF METFORMIN HYDROCHLORIDE AND PIOGLITAZONE HYDROCHLORIDE IN TABLET DOSAGE FORM BY RP-HPLC

Authors :

Madugula Sirisha, Malla Pavithra Pavani, Mamidi Sravani, Mariserla Devayani, Miriyala Renuka Swathi, Mutyala Lohith, Chandaka Madhu

Abstract :

A simple, sensitive and rapid reverse phase high performance liquid chromatographic method was developed for the estimation of Metformin Hcl (MET) and Pioglitazone (PIO) in pure and in pharmaceutical dosage forms. A BDS Hypersil C18column (250×4.6mm, 5μ) was used with a mobile phase containing a mixture of Acetonitrile and Potassium di hydrogen ortho phosphate buffer (pH-3) in the ratio of 50: 50. The flow rate was 1ml/min and effluents were monitored at 238nm and eluted at 2.81min (MET) and 4.57min (PIO). Calibration curve was plotted with a range from 40-240 µg / ml for MET and 12-72 µg / ml for PIO. The assay was validated for the parameters like accuracy, precision, specificity, robustness, ruggedness and system suitability parameters. The proposed method can be useful in the routine analysis for the determination on metformin and pioglitazone in pharmaceutical dosage forms.
Keywords: MetforminHydrochloride, PioglitazoneHydrochloride, Reverse phase HPLC, Pharmaceutical dosage forms, simultaneous estimation.

Cite This Article:

Please cite this article in press Chandaka madhu et al, Analytical Method Development And Method Validation For The Simultaneous Estimation Of Metformin hydrochloride And Pioglitazone hydrochloride In Tablet Dosage Form By RP-HPLC., Indo Am. J. P. Sci, 2023; 10 (06).

Number of Downloads : 10

References:

1. Lalhriatpuii TC, Kawathekar N,Derivative Spectrophotometric Estimationof Pioglitazone and Metformin hydrochloride, Ind Drugs 2005; 42: 740-743.
2. Ajithdas A, Nancy K, Simultaneous Estimation of Metformin hydrochloride and Glipizide in Solid Dosage Forms by Ultraviolet Spectrophotometry, Ind Drugs 2000; 37: 533-536.
3. Vasudevan M, Ravi J, Ravisankar S, Suresh B, Ion-Pair Liquid Chromatography
4. Technique for the Estimation of Metformin in its Multi Component Dosage Forms,
5. J. Pharm Biomed Anal, 2001; 25: 77-84.
6. Sakalgaonkar AA, Mirgane SR, Arbad BR, Validated High Performance Liquid Chromatographic Stability Indicating Method for Analysis of Metformin HCl,
7. Int. J. App Environ Sci 2008; 3: 65–73.
8. Saad SM Hassan, Wagiha H Mahmoud, Mohamed AF Elmosallamy, Abdel Hammeed M Othman, Determination of Metformin in Pharmaceutical Preparations using Potentiometry, Spectrofluorimetry and UV–Visible Spectrophotometry,
9. AnalChim Acta 1999; 378: 299-311.
10. Wanjari DB, Gaikwad NJ, Stability Indicating RP-HPLC Method for Determination of Pioglitazone from Tablets, Ind J. Pharm Sci 2005; 67: 256-258.
11. Kenji Yamashita, Hiromi Murakami, Teruaki Okuda, Michio Motohashi, High-Performance Liquid Chromatographic Determination of Pioglitazone and its Metabolites in Human Serum and Urine, J. Chromatogr B 1996; 677: 141-146.
12. Zhong WZ, Williams MG, Simultaneous Quantitation of Pioglitazone and its Metabolites in Human Serum by Liquid Chromatography and Solid Phase 166 Extraction, J. Pharm. Biomed. Anal 1996; 14: 465-473.
13. Kolte BL, Raut BB, Deo AA, Bagool MA, Shinde DB, Simultaneous High- Performance Liquid Chromatographic Determination of Pioglitazone and Metformin in Pharmaceutical-Dosage Form, J. Chrom Science 2004; 42: 27-31.
14. Shankar Madhira B, Modi Vaibhav D, Shah Dimal A, Bhatt Kashyap K, Mehta Rajendra S, Geetha Madhira et al.,Estimation of Pioglitazone hydrochloride and Metformin hydrochloride in tablets by Derivative Spectrophotometry and Liquid Chromatographic Methods, J. J. AOAC International 2005; 88: 1167-1172.
15. Sahoo PK, Sharma R, Chaturvedi SC, Simultaneous Estimation of Metformin hydrochloride and Pioglitazone hydrochloride by RP-HPLC method from combined tablet dosage form, Ind J.Pharm Sci 2008; 70: 383-386.
16. K.S.Lakshmi,T.Rajesh,Simultaneous,Determinationof Metformin and Pioglitazone by Reversed phase HPLC in Pharmaceutical dosageforms, International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 1, Issue 2, Oct-Dec. 2009.

</div8