Volume : 10, Issue : 07, July – 2023
Title:
16.ADEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF METFORMIN AND GLIPIZIDE IN BULK AND TABLET DOSAGE FORM
Authors :
Samana Pavan Kumar*, Mrs. M. Vineela, Mrs. Raj Khiani
Abstract :
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Glipizide and Metformin, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Zorbax C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol: Phosphate Buffer pH 3.9 (55:45v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 255nm. The retention time of the Glipizide and Metformin was 2.061, 2.462 ±0.02min respectively. The method produce linear responses in the concentration range of 1-5µg/ml of Glipizide and 100-500µg/ml of Metformin. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Keywords: Glipizide, Metformin, RP-HPLC, validation.
Cite This Article:
Please cite this article in press Samana Pavan Kumar et al, Development And Validation Of RP-HPLC Method For The Simultaneous Estimation Of Metformin And Glipizide In Bulk And Tablet Dosage Form., Indo Am. J. P. Sci, 2023; 10 (07).
Number of Downloads : 10
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