Volume : 10, Issue : 09, September – 2023
Title:
32.A NEW METHOD WAS ESTABLISHED FOR SIMULTANEOUS ESTIMATION OF ELBASVIR AND GRAZOPREVIR BY RP-HPLC METHOD
Authors :
Vanapalli Sindhu, Sumalatha Chepyala
Abstract :
Objective: A simple, Accurate, precise method was developed for the simultaneous estimation of the Elbasvir and Grazoprevir in pharmaceutical dosage form.
Methods: Chromatogram was run through symmetry C18, 250 x 4.6 mm, 5µm. Mobile phase containing 0.1% Ortho Phosphoric acid: Acetonitrile, (55:45, v/v) was pumped through column at a flow rate of 1 ml/min. Temperature was maintained at Ambient. Optimized wavelength for Elbasvir and Grazoprevir was 260 nm.
Results: Retention time of Elbasvir and Grazoprevir were found to be 3.848 min and 2.313 min. The % purity of Elbasvir and Grazoprevir was found to be 100.4 % and 100.2 % respectively. The system suitability parameters for Elbasvir and Grazoprevir such as theoretical plates and tailing factor were found to be 3568.30 and 4836.12. The linearity study for Elbasvir and Grazoprevir was found in concentration range of 12.5 μg-75 μg and 25 μg-150 μg and correlation coefficient (r2) was found to be 0.999 and 0.999, % mean recovery was found to be 100.19 % and 100.84 %, %RSD for repeatability was 0.75 and 0.36 %. The precision study was precise, robust and repeatable. LOD value was 0.082 and 0.357, and LOQ value was 1.05 and 0.23 respectively
Conclusion: The results of study showed that the proposed RP‐HPLC method is a simple, accurate, precise, rugged, robust, fast and reproducible, which may be useful for the routine estimation of Elbasvir and Grazoprevir in pharmaceutical dosage form.
Keywords: Elbasvir, Grazoprevir, RP‐HPLC, Simultaneous estimation.
Cite This Article:
Please cite this article in press Vanapalli. Sindhu et al, A New Method Was Established For Simultaneous Estimation Of Elbasvir And Grazoprevir By RP-HPLC Method, Indo Am. J. P. Sci, 2023; 10 (09).
Number of Downloads : 10
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