Volume : 11, Issue : 05, May – 2024
Title:
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF DICLOFENAC Na AND PARACETAMOLIN BULK AND PHARMACEUTICAL DOSAGE FORM BY USING RP-HPLC
Authors :
Katravath Sumithra *, Reshma P
Abstract :
A new simple, accurate, economic, rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Paracetamol and Diclofenac Na, in its pure form as well as in pharmaceutical dosage form. Chromatography was carried out on X bridge C18 (4.6×150mm) 5 µ column using a mixture of Methanol: Phosphate Buffer pH-3.6 (30:70v/v)as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 260nm. The retention time of the Paracetamol and Diclofenac Na was2.669, 3.855±0.02min respectively. The method produces linear responses in the concentration range of 10-50µg/ml of Paracetamol and 10-50µg/ml of Diclofenac Na. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Keywords: Paracetamol and Diclofenac Na, RP-HPLC, Validation.
Cite This Article:
Please cite this article in press Katravath Sumithra et al, Analytical Method Development And Validation For The Simultaneous Estimation Of Diclofenac Na And Paracetamol in Bulk And Pharmaceutical Dosage Form By Using RP-HPLC .,Indo Am. J. P. Sci, 2024; 11 (5).
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