Volume : 10, Issue : 06, June – 2023
Title:
16.DEVELOPMENT OF RP-HPLC METHOD FOR THE ANALYSIS OF LORATADINE AND ITS APPLICATION IN THE EVALUATION OF MARKETED PREPARATION
Authors :
B. Lakshmi Kalyani*, V. Nikhila, J. Raga Swetha, N. Swarna, B. Vidhya, G. Srujana
Abstract :
A simple, selective, rapid and precise reversed-phase high-performance liquid chromatographic method for analysis of Loratadine in bulk and tablet dosage form has been developed and validated. Chromatography was performed on a Waters C18, 5µm particle size, 25cmx4.6mm i.d. with ACN : Methanol = 85 : 15 v/v as mobile phase at a flow rate of 1.0 mL min−1. UV detection was performed at 212 nm. Total run time was 10 min. Loratadine were eluted with retention time of 5.776 minutes. The method was validated for accuracy, precision, linearity, specificity, and sensitivity in accordance with USP and ICH guidelines. Validation revealed the method is specific, rapid, accurate, precise, reliable, and reproducible. Calibration plots were linear over the concentration ranges 0-28μgmL−1 for Loratadine. Limit of detection were 0.06mg mL−1 and limit of quantification were 0.18mg mL−1 for Loratadine. The high recovery and low coefficients of variation confirm the suitability of the method for analysis of the drug in tablet dosage form. The validated method was successfully used for quantitative analysis of Loratin in Loratadine tablets.
Keywords: Analytical Method Development, Estimation, Loratadine, Linearity, RP-HPLC.
Cite This Article:
Please cite this article in press B. Lakshmi Kalyani et al, Development Of RP-HPLC Method For The Analysis Of Loratadine And Its Application In The Evaluation Of Marketed Preparation., Indo Am. J. P. Sci, 2023; 10 (06).
Number of Downloads : 10
References:
1. “The Nobel Prize in Chemistry 1952”. Nobelprize.org. Retrieved 25 August 2016.
2. McMurry, John (2011). Organic chemistry: with biological applications (2nd ed.). Belmont, CA: Brooks/Cole.
3. IUPAC. Compendium of Chemical Terminology, 2nd ed. (the “Gold Book”). Compiled by
4. D. McNaught and A. Wilkinson. Blackwell Scientific Publications, Oxford (1997).
5. Hostettmann, K; Marston, A; Hostettmann, M (1998). Preparative Chromatography Techniques Applications in Natural Product Isolation (Second ed.). Berlin, Heidelberg: Springer Berlin Heidelberg.
6. https://microbenotes.com/chromatography-principle-types-and-applications/
7. https://www.scribd.com/document/178065088/Chromatography-Classification-Principle- of-Working-andSelected-Techniques.
8. https://chemistry.tutorvista.com/analytical-chemistry/uses-of-chromatography.html
9. Karlsson E, Ryden L, Brewer J Protein purification. Principles, High Resolution Methods, and Applications. Ion exchange chromatography. 2nd ed. New York: Wiley; 1998.
10. Amercham Biosciences. Ion Exchange chromatohgraphy, Principles and methods, Amercham Pharmacia. Biotech SE. 2002;751
11. Walls D, Loughran ST. Protein chromatography:Methods and protocols, methods in molecular biology. 2011;681
12. Helmut D. Gel Chromatography, gel filtration, gel permeation, molecular sieves:a laboratory hand book. Springer-Verlag; 1969.
13. Determann H. Gel chromatography gel filtration, gel permeation, molecular sieves:a laboratory handbook. Chapter 2. Materials and Methods. 2012
14. Wilchek M, Chaiken I. An overview of affinity chromatography in affinity chromatography–Methods and protocols. Humana Press. 2000:1–6.
15. Firer MA. Efficient elution of functional proteins in affinity chromatography. J Biochem Biophys Methods. 2001;49:433–42.
16. Stoddard JM, Nguyen L, Mata-Chavez H, Nguyen K. TLC plates as a convenient platform for solvent-free reactions. Chem Commun (Camb) 2007;12:1240–1.