Volume : 10, Issue : 06, June – 2023
Title:
50.DEVELOPMENT AND VALIDATION OF CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TRANDOLAPRIL AND VERAPAMIL IN PURE FORM AND PHARMACEUTICAL DOSAGE FORM BY USING HPLC
Authors :
J. Kalpana*, S.S. Phanindra
Abstract :
A new, simple and accurate, precise RP-HPLC method was developed for simultaneous determination of Trandolapril and Verapamil in bulk and in combined pharmaceutical dosage form. The separation of Trandolapril and Verapamil was achieved within 8 minutes on an Agilent Zorbax (C18) (150mm x 4.6mm, 5µm) column using Methanol: Acetate Buffer pH-3.8 (24:76v/v) as the mobile phase. Detection was carried out using wavelength at 262nm. The method showed adequate sensitivity concerning linearity, accuracy and precision over the range 100-500μg/ml and 30-70μg/ml for Trandolapril and Verapamil, respectively. Careful validation proved advantages of high sensitivity, accuracy, precision, selectivity, robust and suitability for quality control laboratories. The developed method was robust as the %RSD was within the range and without effecting system suitability parameters. The proposed method is suitable for simultaneous determination of Trandolapril and Verapamil in bulk and pharmaceutical dosage form.
Keywords: Trandolapril and Verapamil, RP-HPLC, Validation, Precision, Robustness, ICH Guidelines.
Cite This Article:
Please cite this article in press J. Kalpana et al, Development And Validation Of Chromatographic Method For The Simultaneous Estimation Of Trandolapril And Verapamil In Pure Form And Pharmaceutical Dosage Form By Using HPLC., Indo Am. J. P. Sci, 2023; 10 (06).
Number of Downloads : 10
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