Volume : 10, Issue : 10, October – 2023
Title:
DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF ECONAZOLE AND TRIAMCINOLONE BY RP- HPLC
Authors :
Puttapaka Shirisha*, Dr.D.Venkata Ramana, Ms. Udaya Bhanu Sri Koppu
Abstract :
A new, simple, rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validation of Econazole and Triamcinolone in its pure form as well as in combined marketed formulation. Chromatography was carried out on a Phenomenex Luna C18 (4.6mm×250mm) 5µm particle size column using a mixture of Methanol: Phosphate Buffer (pH-4.2) (37:63% v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 275nm. The retention time of the Econazole and Triamcinolone was found to be was 2.133, 3.692 ± 0.02min respectively. The method was validated according to ICH guidelines for linearity, sensitivity, accuracy, precision, specificity and robustness. The method produce linear responses in the concentration range of 20-60mg/ml of Econazole and 10-30mg/ml of Triamcinolone. The inter-day and intra-day precisions were found to be within limits. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Keywords: Econazole and Triamcinolone, RP-HPLC, Validation, Accuracy, Precision.
Cite This Article:
Please cite this article in press Puttapaka Shirisha et al, Development And Validation Of Analytical Method For Simultaneous Estimation Of Econazole And Triamcinolone By Rp- Hplc, Indo Am. J. P. Sci, 2023; 10 (10).
Number of Downloads : 10
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