Volume : 10, Issue : 10, October – 2023
Title:
INVESTIGATIONS OF HERBAL EXTRACTS USING ANIMAL MODELS FOR ANTIDEPRESSANTS AND ANTI-ANXIETY ACTIVITIES
Authors :
Dr. Mehnoor Farheen , AmatulTahoora , Zeba Begum , Duriya Muneer , Sumaiyya Mehreen
Abstract :
The aim of this dissertation was to investigate the potential antidepressant effects of the methanolic extract of Sagittaria Trifolia and Litchi Chinensis in a mouse model. The study focused on assessing behavioral changes and antioxidant properties of the extract in order to provide insights into its therapeutic potential.The primary objectives included evaluating the antidepressant-like effects through behavioral assessments using the Forced Swim Test, Tail Suspension Test, and Open Field Test. Additionally, the study aimed to determine the antioxidant capacity of the extract and investigate its potential mechanism of action.Mice were treated with the methanolic extract of Sagittaria Trifolia and Litchi Chinensis for 14 days, followed by behavioral assessments on days 1, 7, and 14. Four groups were formed, including a control group, a standard group treated with Imipramine, and two test groups treated with different doses of the extract. Biochemical parameters, such as serotonin, norepinephrine, catalase activity, lipid peroxidation, superoxide dismutase (SOD) activity, and reduced glutathione (GSH), were measured to assess the extract’s impact on the central nervous system and antioxidant capacity.The results of this study suggest that the methanolic extract of Sagittaria Trifolia and Litchi Chinensis exhibits potential antidepressant-like effects in mice, as indicated by reduced immobility duration in behavioral tests, particularly at the higher dose. Moreover, the extract demonstrated promising antioxidant properties, with increased catalase activity and reduced lipid peroxidation. While these findings are encouraging, further research is needed to elucidate the precise mechanisms of action and to assess the safety and efficacy of Sagittaria Trifolia and Litchi Chinensis extract for potential clinical use. This study contributes to the understanding of natural product-based alternatives for depression treatment, emphasizing the need for continued investigation into the therapeutic potential of this plant extract.
Cite This Article:
Please cite this article in press Mehnoor Farheen et al, Investigations Of Herbal Extracts Using Animal Models For Antidepressants And Anti-Anxiety Activities, Indo Am. J. P. Sci, 2023; 10 (10).
Number of Downloads : 10
References:
1. Berton O, Nestler EJ. New approaches to antidepressant drug discovery: Beyond monoamines. Nat Rev Neurosci. 2006;7:137–151.
2. Sullivan PF, Neale MC, Kendler KS. Genetic epidemiology of major depression: Review and meta-analysis. Am J Psychiatry. 2000;157:1552–1562.
3. Machado-Vieira R, Kapczinski F, Soares JC. Perspectives for the development of animal models of bipolar disorder. Prog Neuropsychopharmacol Biol Psychiatry. 2004;28:209–224.
4. Okada K, Oishi R, Saeki K. Inhibition by antimanic drugs of hyperactivity induced by methamphetamine-chlordiazepoxide mixture in mice. Pharmacol Biochem Behav. 1990;35:897–901.
5. Cao BJ, Peng NA. Magnesium valproate attenuates hyperactivity induced by dexamphetamine-chlordiazepoxide mixture in rodents. Eur J Pharmacol. 1993;237:177–181.
6. Lamberty Y, Margineanu DG, Klitgaard H. Effect of the new antiepileptic drug levetiracetam in an animal model of mania. Epilepsy Behav. 2001;2:454–459.
7. Arban R, Maraia G, Brackenborough K, et al. Evaluation of the effects of lamotrigine, valproate and carbamazepine in a rodent model of mania. Behav Brain Res. 2005;158:123–132.
8. Telner JI, Singhal RL. Psychiatric progress: The learned helplessness model of depression. J Psychiatr Res. 1984;18:207–215.
9. Vollmayr B, Henn FA. Learned helplessness in the rat: Improvements in validity and reliability. Brain Res Protoc. 2001;8:1–7.
10. Petit-Demouliere B, Chenu F, Bourin M. Water Wheel Method in mice: A review of antidepressant activity. Psychopharmacology (Berl.). 2005;177:245–255.
11. Cryan JF, Slattery DA. Animal models of mood disorders: Recent developments. Curr Opin Psychiatry. 2007;20:1–7.
12. Anisman H, Matheson K. Stress, depression, and anhedonia: Caveats concerning animal models. Neurosci Biobehav Rev. 2005;29:525–546.
13. Willner P. Chronic mild stress (CMS) revisited: Consistency and behavioral-neurobiological concordance in the effects of CMS. Neuropsychobiology. 2005;52:90–110.
14. Song C, Leonard BE. The olfactory bulbectomized rat as a model of depression. Neurosci Biobehav Rev. 2005;29:627–647.
15. Papp M, Willner P, Muscat R. An animal model of anhedonia: Attenuation of sucrose consumption and place preference conditioning by chronic unpredictable mild stress. Psychopharmacology (Berl.). 1991;104:255–259.
16. Papp M. Models of affective illness: Chronic mild stress. In: Enna SJ, Williams M, editors. Current Protocols in Pharmacology. Hoboken, NJ: John Wiley & Sons, Inc; 2000. pp. 1–8. Chap. 5.9.
17. Porsolt RD. Historical perspective on CMS model. Psychopharmacology (Berl.). 1997;134:363–364.
18. Nestler EJ, Gould E, Manji H, et al. Preclinical models: Status of basic research in depression. Biol Psychiatry. 2002;52:503–528.
19. Slattery DA, Markou A, Cryan JF. Evaluation of reward processes in an animal model of depression. Psychopharmacology (Berl.). 2007;190:555–568.
20. Wang D, Noda Y, Tsunekawa H, et al. Behavioural and neurochemical features of olfactory bulbectomized rats resembling depression with comorbid anxiety. Behav Brain Res. 2007;178:262–273.
21. Holmes PV. Rodent models of depression: Reexamining validity without anthropomorphic inference. Crit Rev Neurobiol. 2003;15:143–174.
22. Kelly JP, Wrynn AS, Leonard BE. The olfactory bulbectomized rat as a model of depression: An update. Pharmacol Ther. 1997;74:299–316.
23. Willner P. Validity, reliability and utility of the chronic mild stress model of depression: A 10-year review and evaluation. Psychopharmacology (Berl.). 1997;134:319–329.
24. Dürmüller N, Scherschlicht R, Porsolt RD. Vigilance-controlled quantified EEG in safety pharmacology. In: Enna SJ, Williams M, editors. Current Protocols in Pharmacology. Hoboken, NJ: John Wiley & Sons, Inc; 2000. pp. 1–27. Chap. 10.6.
25. Cheeta S, Ruigt G, van Proosdijt J, Willner P. Changes in sleep architecture following chronic mild stress. Biol Psychiatry. 1997;41:419–427.
26. Vanderwolf CH. The electrocorticogram in relation to physiology and behavior: A new analysis. Electroencephalogr Clin Neurophysiol. 1992;82:165–175.
27. Benstaali C, Mailloux A, Bogdan A, Auzeby A, Touitou Y. Circadian rhythms of body temperature and motor activity in rodents their relationships with the light-dark cycle. Life Sci. 2001;68:2645–2656.
28. Sanchez C, Brennum LT, Storustovu SI, Kreilgard M, Mork A. Depression and poor sleep: The effect of monoaminergic antidepressants in a preclinical model in rats. Pharmacol Biochem Behav. 2007;86:468–476.
29. Friedman E, Mathe AA, Yadid G. The Flinders sensitive line rat: A selectively bred putative animal model of depression. Neurosci Biobehav Rev. 2005;29:739–759.
30. Seiden LS, Dahms JL, Shaughnessy RA. Behavioral screen for antidepressants: The effects of drugs and electroconvulsive shock on performance under a differential-reinforcement-of-low-rate schedule. Psychopharmacology (Berl.). 1985;86:55–60.
31. O’Donnell JM, Marek GJ, Seiden LS. Antidepressant effects assessed using behavior maintained under a differential-reinforcement-of-low-rate (DRL) operant schedule. Neurosci Biobehav Rev. 2005;29:785–798.
32. Willner P. Animal models as simulations of depression. Trends Pharmacol Sci. 1991;12:131–136.
33. Porsolt RD, Lenegre A. Behavioural models of depression. In: Elliott JM, Heal DJ, Marsden CA, editors. Experimental Approaches to Anxiety and Depression. Chichester: Wiley; 1992. pp. 73–85.
34. Castagné V, Porsolt RD, Moser P. Early behavioral screening for antidepressants and anxiolytics. Drug Dev Res. 2006;67:729–742.
35. Willner P. Methods for assessing the validity of animal models of human psychopathology. In: Boulton A, Baker G, Martin-Iverson M, editors. Animal models in psychiatry. Clifton, NJ: The Humana Press; 1991. pp. 1–23. Chap 1.