Volume : 11, Issue : 02, February – 2024

Title:

LARVICIDAL ACTIVITY OF INDIGENOUS PLANT AQUEOUS EXTRACTS ON MALARIAL VECTOR, ANOPHELES SUBPICTUS GRASSI (DIPTERA: CULICIDAE)

Authors :

B.Palani , V.K. Sivakumar, A. Thalavaipandian , V. Jayakumar, N. Arjun, M. Vignesh, R. Prithika, R. Thulasi, S.Renuka, C.Kothai, G. Elango**

Abstract :

Mosquito control is facing a threat due to the emergence of resistance to synthetic insecticides. Insecticides of botanical origin may serve as suitable alternative bio control techniques in the future. The purpose of the present study is to assess the effect of leaf cold andHot water extracts of Ocimum basilicum,Ocimum americanum,Cleome gynandra and Passiflora foetida. All plant extracts showed moderate toxic effect on larvae after 24 h of exposure at 1,000 ppm;however, the highest larvicidal mortalityactivity was observed after 48 hcold water extracts of C.gynandra (LC50=135.86, 155.58 and121.61ppm), (LC90=594.12 720.87and 471.34 ppm ) andP.foetida, (LC50=140.60, 161.38 and126.72ppm), (LC90=628.20 and 784.14–498.75ppm) and hot water extracts of O. americanum(LC50=85.10, 89.40 and 67.19 ppm),(LC90 =364.12, 468.20–375.12ppm), and C.gynandra, (LC50=167.82, 189.75and148.12ppm) (LC90 = 630.41, 782.11and492.94 ppm), and the overall findings of the present study have shown that the leaf aqueous plant extracts have the potential to be used as an ideal ecofriendly approach for the control of the A. subpictus. The screening results suggest that the cold water extract of C.gynandra, and hot water extract of O. americanumare promising in mosquito control.

Key Words:Anopheles subpictus, plant extracts, cold and hot water, larvicidal activity

Cite This Article:

Please cite this article in press Gandhi Elango et al., Larvicidal Activity Of Indigenous Plant Aqueous Extracts On Malarial Vector, Anopheles Subpictus Grassi (Diptera: Culicidae), Indo Am. J. P. Sci, 2024; 11 (02).

Number of Downloads : 10

References:

1. Amerasinghe PH, Amerasinghe F P (1999). Multiple host feeding in field populations of Anopheles culicifaciesand A.subpictusin Sri Lanka. Medical and Veterinary Entomology13(2): 124–131.
2. Bagavan A, Kamaraj C, Rahuman AA, Elango G, Zahir AA, Pandiyan G (2008). Evaluation of larvicidal and nymphicidal potential of plant extracts against Anopheles subpictus Grassi, Culex tritaeniorhynchus Giles and Aphis gossypii Glover. ParasitolRes. ;104:1109
3. Chokechaijaroenporn O, Bunyapraphatsara N, Kongchuensin S (1994) Mosquito repellent activities of Ocimum volatile oils. Phytomedicine 1994, 1, 135–139
4. Choochote W, Kanjanapothi D, Panthong A, Taesotikul T, Jitpakdi A, Chaithong U, Pitasawat B (1999) Larvicidal, adulticidal and repellent effects of Kaempferia galanga. Southeast Asian J Trop Med Public Health 30(3):470–476
5. Chowdhury N, Ghosh A, Chandra G (2008) Mosquito larvicidal activities of Solanum villosum berry extract against the dengue vector Stegomyia aegypti. BMC Complement Altern Med 3 (8):10
6. Dharmagadda, VS, Naik, SN, Mittal, PK, Vasudevan P (2005) Larvicidal activity of Tagetes patula essential oil against three mosquito species. Bioresour. Technol. 96 (11), 1235–1240
7. Elango G, Rahuman AA, Bagavan A, Kamaraj C, Zahir AA, Venkatesan C (2009) Laboratory study on larvicidal activity of indigenous plant extracts against Anopheles subpictus and Culex tritaeniorhynchus. Parasitol Res 104:1381–1388
8. Flora and Fauna Web. Ocimum americanum. (2022). Available online: https://www.nparks.gov.sg/florafaunaweb/flora/5/0/5057 (accessed on 1 March 2023).
9. Ganesan M and Sornkanok V (2023) Chemical compositions, antioxidant, antimicrobial, and mosquito larvicidal activity of Ocimumamericanum L. and Ocimum basilicum L. leafessential oilsBMC Complementary Medicine and Therapies23: 390
10. Green, MM, Singer JM, Sutherland DJ, Hibben, CR (1991) Larvicidal activity of Tagetes minuta(marigold) toward Aedes aegypti. J. Am. Mosq. Control Assoc. 7 (2), 282–286
11. Gubler DJ (1998) Resurgent vector-borne diseases as a global health problem. Emerg Infect Dis 4:1–9
12. Huimei L, Sook Y, LeeLai Y H, Nam W S (2023) Mosquito Larvicidal Activity and Cytotoxicity of the Extracts of Aromatic Plants from Malaysia Insects. 2023 Jun; 14(6): 512
13. Jantan I, Yalvema, MF, Ahmad NW, Jamal JA (2005) Insecticidal activities of the leafoils of eight Cinnamomum species against Aedes aegypti and Aedes albopictus Pharmaceutical Biology43: 526–532.
14. Kager PA (2002) Malaria control: constraints and opportunities.Trop Med Int Health 7:1042–1046
15. Kannathasan K, Senthilkumar A, Chandrasekaran M, Venkatesalu V (2007) Differential larvicidal efficacy of four species of Vitex against Culex quinquefasciatus larvae. Parasitol Res 101(6):1721–1723
16. Kannathasan K, Senthilkumar A, Venkatesalu V, Chandrasekaran M (2008) Larvicidal activity of fatty acid methyl esters of Vitex species against Culex quinquefasciatus. Parasitol Res 103(4):999–100
17. Komalamisra N, Trongtokit Y, Rongsriyam Y, Apiwathnasorn C (2005) Screening for larvicidal activity in some Thai plants against four mosquito vector species. Southeast Asian J Trop Med Public Health 36(6):1412–1422
18. Kondrachine AV (1992) Malaria in WHO Southeast Asia Region. Indian J Malariol; 29: 129–60
19. Kumar A, Dutta GP (1987) Indigenous plant oils as larvicidal agent against Anopheles stephensi mosquitoes. Curr Sci 56:959–960
20. Kumar A, Valecha N, Jain T, Aditya P (2007) Dash burden of malaria in India retrospective and prospective view. The American Journal of Tropical Medicine and Hygiene77(6): 69–78
21. Macêdo ME, Consoli RA, Grandi TS, dos Anjos AM, de Oliveira AB, Mendes NM, Queiróz RO, Zani CL (1997) Screening of Asteraceae (Compositae) plant extracts for larvicidal activity against Aedes fluviatilis (Diptera:Culicidae). Mem Inst Oswaldo Cruz 92(4):565–570
22. Motaal AA, Ezzat SM, Haddad PS. Determination of bioactive markers in Cleome droserifolia using cell-based bioassays for antidiabetic activity and isolation of two novel active compounds. Phytomedicine. 2011;19:38–41
23. Mullai K, Jebanesan A, Pushpanathan T (2008) Mosquitocidal and repellent activity of the leaf extract of Citrullus vulgaris (cucurbitaceae) against the malarial vector, Anopheles stephensiliston (dipteraculicidae). Eur Rev Med Pharmacol Sci 12(1):1–7
24. Perich MJ, Wells C, Bertsch W, Tredway KE, (1995). Isolation of the insecticidal components of Tagetes minuta (Compositae) against mosquitolarvae and adults. J. Am. Mosq. Control Assoc. 11 (3), 307–310
25. Prabhavathi O, Yuvarajan R, Natarajan D (2016 )Mosquitocidal properties of Ocimum canum Sims (Lamiaceae) leaf extracts against dengue vector Aedes aegypti L. (Diptera: Culicidae). J. Entomol. Acarol. Res. 2016, 48, 345–354
26. Rahuman AA, Gopalakrishnan G, Ghouse BS, Arumugam S, Himalayan B (2000) Effect of Feronia limonia on mosquito larvae. Fitoterapia 71(5):553–555
27. Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K, Bagavan A (2008) Mosquito larvicidal activity of isolated compounds from the rhizome of Zingiber officinale. Phytother Res 22(8):1035–1039
28. Rahuman AA, Venkatesan P (2008) Larvicidal efficacy of five cucurbitaceous plant leaf extracts against mosquito species. Parasitol Res 103:133–139
29. Reddy PJ, Krishna D, Murthy US, Jamil K (1992) A microcomputer FORTRAN program for rapid determination of lethal concentration of biocides in mosquito control. CABIOS 8:209–213
30. Ross MSF, Brian KR (1977) An introduction to pharmacy. Pitman, Bath, Great Britain
31. Saravanan KS, Periyanayagam K, Ismail M (2007) Mosquito larvicidal properties of various extract of leaves and fixed oil from the seeds of Caesalpinia bonduc (L) Roxb. J Commun Dis 39(3):153–157
32. Sarin R, (2004) Insecticidal activity of callus culture of Tagetes erecta. Fitoterapia 75 (1), 62– 64
33. WHO (2008) World Malaria Report. Disease Burden in SEA Region. Regional office for South East Asia, New Delhi
34. WHO (1996) Report of the WHO informal consultation on the evaluation on the testing of insecticides CTD/WHO PES/IC/96.1: p 69
35. WHO (2007) Anopheline species complexes in South and South-East Asia, vol 57. Regional office for South East Asia, New Delhi, pp 771 22–32
36. Zaim M, Guillet P (2002) Alternative insecticides: an urgent need. Trends Parasitol 18:161–163