Volume : 11, Issue : 02, February – 2024
Title:
FLOURIMETRY – REVIEW ARTICLE
Authors :
E. Naga Deepthi*, U. Manjula, P. Bhargavi, B. Vamshi Krishna, B.V. Ramana,
Abstract :
Flourimetry is a measurement of fluorescence intensity at a particular wavelength involving the phenomena of emission of radiation when there is transition from singlet excited state to singlet ground state. The wavelength of absorbed radiation is called as excitation wavelength and that of emitted radiation is called as emission wavelength. Phosphorescence is a specific type of photo luminescence related to fluorescence. In fluorescence, the molecules of the substance from the ground state excite to singlet state and return back to the ground state. In phosphorescence molecules of the substance in triplet excited state return to ground state. The deuterium lamp was used as a source of light, primary and secondary filter are built in it, quartzs,cuvettes was used for handling the sample come photo multiplier tube was employed as the detector LED (Fluorimeter model LF-2) (QUANTALASE India). The percentage fluorescence intensity was determined. The results were analysed for precision, accuracy, specificity, robustness and ANOVA test was performed. It gave accurate results in microgram concentration in solution.
Keywords: Flourescence, phosphorescence, deuterium lamp, photomultiplier tube, ANOVA,
Cite This Article:
Please cite this article in press Elluru. Naga Deepthi et al., Flourimetry – Review Article, Indo Am. J. P. Sci, 2024; 11 (02).
Number of Downloads : 10
References:
1. Valeur Bernard, Berberan-Santos, Mário N., A Brief History of Fluorescence and Phosphorescence before the Emergence of Quantum Theory, 88(6) (2011) 731–738.
2. BK. Sharma, Instrumental methods of chemical analysis, Molecular fluorescence spectroscopy, (2011) S537-S568.
3. Dr. S. Ravishankar, Text book of pharmaceutical analysis: Fluorimetry, edition 4, 3- 18
4. Valeur, Bernard, Berberan-Santos, Mario Molecular Fluorescence: Principles and Applications, Wiley-VCH. ISBN 978-3-527- 32837-6. p. 64(2012).
5. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, edition 3, Springer, New York, (2006) 954.
6. Aamir Shahzad, Gottfried Köhler, Martin Knapp, Erwin Gaubitzer, Martin Puchinger, Michael Edetsberger, Emerging applications of fluorescence spectroscopy in medical microbiology field, Journal of Translational Medicine 7 (2009) 99
7. SKOOG .Principles of Instrumental Analysis.
8. Practical pharmaceutical chemistry by A.H.BECKETT& J.B.STENLAKE Panigrahi SK, Mishra AK.
9. Ndlovu LA. Warning clothing-study of fluorescenece fading in UV irradiation
(Doctoral dissertation, Technická Univerzita v Liberci).
10. Launay JP, Verdaguer M. Electrons in molecules: from basic principles to molecular electronics. Oxford University Press, USA; 2014.
11. Itoh T. Fluorescence and phosphorescence from higher excited states of organic molecules. Chemical reviews. 2012 Aug 8;112(8):4541-68.
12. Robinson DR, Jencks WP. The effect of compounds of the urea-guanidinium class on the activity coefficient of acetyltetraglycine ethyl ester and related compounds1. Journal of the American Chemical Society. 1965 Jun;87(11):2462-70.
13. Callis PR. Binding phenomena and fluorescence quenching. I: Descriptive quantum principles of fluorescence quenching using a supermolecule approach. Journal of Molecular Structure. 2014 Dec 5;1077:14-21.
14. Sudharshan N, Swetha V. UV-VISIBLE SPECTROSCOPY: A COMPREHENSIVE REVIEW ON INSTRUMENTATION..
15. Ulrich A, Born M, Koops HW, Bluhm H, Jüstel T. Infrared, Light, Ultraviolet, S4 (pp. 303-354). Berlin, Heidelberg: Springer Berlin Heidelberg.
16. Ayres RU, Ayres RU. The History of Artificial Light. The History and Future of Technology: Can Technology Save Humanity from Extinction?. 2021:339-64.
17. Kim JJ. Light Sources Excited by Micro-wave High Intensity Discharges. InThe 10th Int, Symp. on Light Sources 2004 Jan 1 (p. 85).
18. Parker KE, Evans DT. Lamp Materials. Lamps and Lighting, ed. JR Coaton and AM Marsden (Arnold, 1997). 2012 Aug 21:119-36.
19. Wu H, Kong D, Ruan Z, Hsu PC, Wang S, Yu Z, Carney TJ, Hu L, Fan S, Cui Y. A transparent electrode based on a metal nanotrough network. Nature nanotechnology. 2013 Jun;8(6):421-5.
20. Reinitz K. Low Energy Electron Position Sensor. IEEE Transactions on Nuclear Science. 1968 Feb;15(1):352-61.
21. Gilmore AM. How to collect National Institute of Standards and Technology (NIST) traceable fluorescence excitation and emission spectra. Fluorescence Spectroscopy and Microscopy: Methods and Protocols. 2014:3-27.
22. Bose A, Thomas I, Abraham E. Fluorescence spectroscopy and its applications: A Review. Int. J. Adv. Pharm. Res. 2018;8(1):1-8.
23. Naresh K. Applications of fluorescence spectroscopy. J. Chem. Pharm. Sci. 2014;974:2115.
24. Birch DJ, Chen Y, Rolinski OJ. Fluorescence. Photonics: Biomedical Photonics, Spectroscopy, and Microscopy. 2015 Feb 23:1-58.