Volume : 11, Issue : 05, May – 2024
Title:
EXTRACTION, PHYTOCHEMICAL SCREENING AND HEPATOPROTECTIVE ACTIVITY OF OPUNTIA FICUS INDICA
Authors :
Priyanka Aathiya, Harshita Jain, Prateek K. Jain, Sunil Kumar Jain, Basant Khare, Arpit Shrivastava
Abstract :
This study investigates the phytochemical composition and pharmacological properties of the hydroalcoholic extract of Opuntia ficus indica (OFI) leaves and fruits. The hydroalcoholic extract of OFI exhibited a yield of 6.2% w/w, which was higher than the petroleum ether extract (2.7% w/w). Phytochemical screening revealed the presence of alkaloids, flavonoids, proteins, carbohydrates, saponins, and diterpenes in the extract. Quantitative analysis indicated a total alkaloid content of 0.352 mg/100 mg and a total flavonoid content of 0.846 mg/100 mg of dried extract. In an acute toxicity study, the extract did not induce any adverse behavioral changes or mortality, confirming its safety profile. The hepatoprotective effects of the ethanolic fruit extract of OFI were evaluated in D-GalN-induced hepatotoxicity in rats. The extract significantly reduced levels of serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), and bilirubin, while increasing albumin levels, compared to the hepatotoxic control group. These effects were dose-dependent, with the 200 mg/kg dose showing the most pronounced effects, comparable to the positive control Silymarin.
Keywords: Opuntia ficus indica, hydroalcoholic extract, phytochemical screening, alkaloids, flavonoids, hepatoprotective, hepatotoxicity, SGPT, SGOT, ALP, bilirubin, albumin, Silymarin, D-GalN-induced hepatotoxicity.
Cite This Article:
Please cite this article in press Priyanka Aathiya et al., Extraction, Phytochemical Screening And Hepatoprotective Activity Of Opuntia Ficus Indica., Indo Am. J. P. Sci, 2024; 11 (05).
Number of Downloads : 10
References:
1. Mahmood N, Piacente S, Pizza C, Burke A, Khan AI, Hay AJ. The anti-HIV activity and mechanisms of action of pure compounds isolated from Opuntia ficus-indica (L.) Mill. J Ethnopharmacol. 2012; 142(2): 374-82.
2. Nasri H, Shirzad H, Baradaran A, Rafieian-Kopaei M. Antioxidant plants and diabetes mellitus. J Res Med Sci. 2015; 20(5): 491-502.
3. Shi M, Huang F, Deng C, Wang Y, Kai G. Bioactive compounds from Opuntia species: A comprehensive review. J Agric Food Chem. 2020; 68(20): 5517-34.
4. Salaritabar A, Darvishi A, Owji SM, Mansouri K, Ghorbani M. Protective effect of Opuntia ficus-indica f. inermis (L.) mill cladodes against carbon tetrachloride-induced oxidative stress, hepatotoxicity, and nephrotoxicity in mice. Biomed Res Int. 2020; Article ID 2868434.
5. Mukherjee PK. Quality Control of Herbal Drugs, 2nd Edition, Business Horizons, 2007; 2-14.
6. Kokate CK. Ed. Practical Pharmacognosy, 4th Edn., Vallabh Prakashan: 1994; 112:120.
7. Fazel Shamsa, Hamidreza Monsef, Rouhollah Ghamooshi, Mohammadreza Verdianrizi. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J Pharm Sci. 2008; 32: 17-20.
8. Geeta Parkhe, Deepak Bharti. Phytochemical Investigation and Determination of Total Phenols and Flavonoid Concentration in Leaves Extract of Vitex trifolia Linn. Journal of Drug Delivery & Therapeutics. 2019; 9(4-A):705-707.
9. Mondal M, Hossain MM, Hasan MR, et al. Hepatoprotective and Antioxidant Capacity of Mallotusrepandus Ethyl Acetate Stem Extract against d-Galactosamine-Induced Hepatotoxicity in Rats. ACS Omega. 2020;5(12):6523-6531. Published 2020 Mar 18. doi:10.1021/acsomega.9b04189.